POU5F1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
The tissue lysate derived from mouse kidney was immunoprobed by POU5F1 polyclonal antibody (Cat # PAB12773) at 1 : 500.
An immunoreactive band is observed around ~34 kDa (1).
This band is abolished by pre-incubation with immunizing peptide (2).
Dot Blot (Peptide)
Dot Blot : 1 ug peptides was blotted onto NC membrane.
A : POU2F1
B : POU2F2
C : POU5F1
Followed by immunoblotting at a 1 : 1000 dilution by :
1 : POU2F1 polyclonal antibody (Cat # PAB12771)
2 : POU2F2 polyclonal antibody (Cat # PAB12772)
3 : POU5F1 polyclonal antibody (Cat # PAB12773)
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.
Immunogen
A synthetic peptide corresponding to internal domain of human, mouse and rat POU5F1.
Host
Rabbit
Theoretical MW (kDa)
39
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~39 KDa of POU5F1. It does not cross react with POU2F1, POU2F2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysate derived from mouse kidney was immunoprobed by POU5F1 polyclonal antibody (Cat # PAB12773) at 1 : 500.
An immunoreactive band is observed around ~34 kDa (1).
This band is abolished by pre-incubation with immunizing peptide (2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptides was blotted onto NC membrane.
A : POU2F1
B : POU2F2
C : POU5F1
Followed by immunoblotting at a 1 : 1000 dilution by :
1 : POU2F1 polyclonal antibody (Cat # PAB12771)
2 : POU2F2 polyclonal antibody (Cat # PAB12772)
3 : POU5F1 polyclonal antibody (Cat # PAB12773) -
Gene Info — POU5F1
Entrez GeneID
5460Gene Name
POU5F1
Gene Alias
MGC22487, OCT3, OCT4, OTF3, OTF4
Gene Description
POU class 5 homeobox 1
Omim ID
164177Gene Ontology
HyperlinkGene Summary
class 5
Other Designations
OTTHUMP00000029292|POU domain, class 5, transcription factor 1|POU-type homeodomain-containing DNA-binding protein|octamer-binding transcription factor-3
-
Gene Info — Pou5f1
-
Gene Info — Pou5f1
-
Interactome
-
Disease
-
Publication Reference
-
Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1.
Zhang Z, Liao B, Xu M, Jin Y.
FASEB Journal 2007 Oct; 21(12):3042.
Application：WB-Tr, Human, Mouse , HEK 293 cells, Mouse embryonic stem cells.
-
Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com