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Last updated: 2016/12/4
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POU5F1 polyclonal antibody

  • Catalog # : PAB12773
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.
  • Immunogen:
  • A synthetic peptide corresponding to internal domain of human, mouse and rat POU5F1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 39
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes ~39 KDa of POU5F1. It does not cross react with POU2F1, POU2F2.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The tissue lysate derived from mouse kidney was immunoprobed by POU5F1 polyclonal antibody (Cat # PAB12773) at 1 : 500.
    An immunoreactive band is observed around ~34 kDa (1).
    This band is abolished by pre-incubation with immunizing peptide (2).
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptides was blotted onto NC membrane.
    A : POU2F1
    B : POU2F2
    C : POU5F1
    Followed by immunoblotting at a 1 : 1000 dilution by :
    1 : POU2F1 polyclonal antibody (Cat # PAB12771)
    2 : POU2F2 polyclonal antibody (Cat # PAB12772)
    3 : POU5F1 polyclonal antibody (Cat # PAB12773)
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5460
  • Gene Name:
  • POU5F1
  • Gene Alias:
  • MGC22487,OCT3,OCT4,OTF3,OTF4
  • Gene Description:
  • POU class 5 homeobox 1
  • Other Designations:
  • OTTHUMP00000029292,POU domain, class 5, transcription factor 1,POU-type homeodomain-containing DNA-binding protein,octamer-binding transcription factor-3
  • Gene Information
  • Gene Name:
  • Pou5f1
  • Gene Alias:
  • Oct-3,Oct-3/4,Oct-4,Oct3,Oct3/4,Oct4,Otf-3,Otf-4,Otf3,Otf3-rs7,Otf3g,Otf4
  • Gene Description:
  • POU domain, class 5, transcription factor 1
  • Other Designations:
  • -
  • Gene Information
  • Gene Name:
  • Pou5f1
  • Gene Alias:
  • -
  • Gene Description:
  • POU class 5 homeobox 1
  • Other Designations:
  • POU domain, class 5, transcription factor 1
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