POU5F1 polyclonal antibody
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More Files
- More Functions
ChIP
ChIP assays were performed using E14Tg2a mouse embryonic stem cells. A titration of the antibody consisting of 1, 2. 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. The Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP-Seq
ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 ug antibody. The figure shows the peak distribution along the complete sequence of mouse chromosome 7 and a 1Mb region containing the UTF1 positive control, and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes.
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of nuclear extracts of human embryonic stem cells.
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against POU5F1. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:100000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to different parts of human POU5F1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:1000-10000)
Western Blot (1:1000)
ChIP (5 ug/CHIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using E14Tg2a mouse embryonic stem cells. A titration of the antibody consisting of 1, 2. 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. The Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).ChIP-Seq
ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 ug antibody. The figure shows the peak distribution along the complete sequence of mouse chromosome 7 and a 1Mb region containing the UTF1 positive control, and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes.Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of nuclear extracts of human embryonic stem cells.Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against POU5F1. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:100000. -
Gene Info — POU5F1
Entrez GeneID
5460Protein Accession#
Q01860Gene Name
POU5F1
Gene Alias
MGC22487, OCT3, OCT4, OTF3, OTF4
Gene Description
POU class 5 homeobox 1
Omim ID
164177Gene Ontology
HyperlinkGene Summary
class 5
Other Designations
OTTHUMP00000029292|POU domain, class 5, transcription factor 1|POU-type homeodomain-containing DNA-binding protein|octamer-binding transcription factor-3
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Interactome
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Disease
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Publication Reference
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Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.
Holm F et al.
PNAS 2015 Dec; 112(50):15444.
Application：ICC, ChIP, Human, Human chronic myeloid leukemia cells, Human embryonic stem cells.
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Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.
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