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Last updated: 2017/1/22
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CDC2 polyclonal antibody

  • Catalog # : PAB12757
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CDC2.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human, mouse and rat CDC2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The A-431 cell lysate resolved onto 12% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with CDC2 polyclonal antibody (Cat # PAB12757, lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human prostate tissue stained with CDC2 polyclonal antibody (Cat # PAB12757) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
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