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Last updated: 2016/12/4
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MAPK1/MAPK3 (phospho T202/Y204) polyclonal antibody

  • Catalog # : PAB12748
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAPK1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of human, mouse, rat and dog MAPK1/MAPK3.
  • Host:
  • Rabbit
  • Reactivity:
  • Dog, Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes MAPK1/MAPK3 (pT202/pY204) with the dual phosphorylation sites. It does not cross-react with non-phosphospecific peptide.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The whole cell lysate derived from UV treated NIH/3T3 was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by MAPK1 (phospho T202/Y204) polyclonal antibody (Cat # PAB12748) at 1 : 500. A dual immunoreactive band around ~ 42/44 kDa is observed.
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
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