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Last updated: 2016/10/16
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MYC tag polyclonal antibody

  • Catalog # : PAB12716
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MYC tag.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 408-452 of human MYC tag.
  • Sequence:
  • Host:
  • Rabbit
  • Specificity:
  • This antibody recognizes MYC tagged fusion proteins.
  • Form:
  • Liquid
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • The cell lysate of 293 with over-expressed MYC tagged recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane.
    Followed by an immune-blotting with MYC tag polyclonal antibody (Cat # PAB12716) at 1 : 1000.
    Lane 2 was pre-incubated by immunizing peptide.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 4609
  • Gene Name:
  • MYC
  • Gene Alias:
  • bHLHe39,c-Myc
  • Gene Description:
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • Gene Summary:
  • The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
  • Other Designations:
  • avian myelocytomatosis viral oncogene homolog,myc proto-oncogene protein,v-myc avian myelocytomatosis viral oncogene homolog
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