The cell lysate derived from conditioned NIH/3T3 cells was immunoprobed by RPS6KB1 (phospho S434) polyclonal antibody (Cat # PAB12645) at 1 : 500. An immunoreactive band is observed around ~ 70kDa (Lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2).
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq