The 4 mg protein of whole tissue lysates derived from human brain were immunoprecipitated by 4 ug of NRP1 (phospho T916) polyclonal antibody (Cat # PAB12639) o/n at 4°C, and probed by the same antibody at 1 : 1000. An immunoreactive band at ~ 130kDa was observed.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : NRP1 (Phospho-specific peptide at pT916). B : NRP1 (Non-phospho phosphopeptide). C : Non-related phospho-specific phophopeptide. Followed were blotted at a 1 : 1000 dilution by : 1. NRP1 (phospho T916) polyclonal antibody (Cat # PAB12639). 2. NRP1 polyclonal antibody (Cat # PAB12638).
NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.[supplied by OMIM