CUL4B polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis of CUL4B expression from cell and tissue extracts with CUL4B polyclonal antibody (Cat # PAB12320).
Lane 1 : female zebrafish tissue lysate.
Lane 2 : MM453 whole cell lysate.
Lane 3 : rat liver tissue lysate.
Lane 4 : rat lung tissue lysate.
Lane 5 : MCF-7 whole cell lysate.
Lane 6 : MM231 whole cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of CUL4B on formalin fixed, paraffin embedded zebrafish body with CUL4B polyclonal antibody (Cat # PAB12320).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CUL4B.
Immunogen
A synthetic peptide corresponding to human CUL4B.
Host
Rabbit
Theoretical MW (kDa)
76.8, 87.6
Reactivity
Human, Rat, Zebra fish
Specificity
Identical to the related rat and mouse sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of CUL4B expression from cell and tissue extracts with CUL4B polyclonal antibody (Cat # PAB12320).
Lane 1 : female zebrafish tissue lysate.
Lane 2 : MM453 whole cell lysate.
Lane 3 : rat liver tissue lysate.
Lane 4 : rat lung tissue lysate.
Lane 5 : MCF-7 whole cell lysate.
Lane 6 : MM231 whole cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of CUL4B on formalin fixed, paraffin embedded zebrafish body with CUL4B polyclonal antibody (Cat # PAB12320). -
Gene Info — CUL4B
Entrez GeneID
8450Gene Name
CUL4B
Gene Alias
DKFZp686F1470, KIAA0695, MRXHF2, MRXSC, SFM2
Gene Description
cullin 4B
Gene Ontology
HyperlinkGene Summary
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023946|OTTHUMP00000023947|cullin-4B
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Interactome
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Pathway
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Publication Reference
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Dioxin receptor is a ligand-dependent E3 ubiquitin ligase.
Ohtake F, Baba A, Takada I, Okada M, Iwasaki K, Miki H, Takahashi S, Kouzmenko A, Nohara K, Chiba T, Fujii-Kuriyama Y, Kato S.
Nature 2007 Mar; 446(7135):562.
Application：IP, WB, Human, LNCaP, MCF-7 cells.
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Mutation in CUL4B, which encodes a member of cullin-RING ubiquitin ligase complex, causes X-linked mental retardation.
Zou Y, Liu Q, Chen B, Zhang X, Guo C, Zhou H, Li J, Gao G, Guo Y, Yan C, Wei J, Shao C, Gong Y.
American Journal of Human Genetics 2007 Mar; 80(3):561.
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Mutations in CUL4B, which encodes a ubiquitin E3 ligase subunit, cause an X-linked mental retardation syndrome associated with aggressive outbursts, seizures, relative macrocephaly, central obesity, hypogonadism, pes cavus, and tremor.
Tarpey PS, Raymond FL, O'Meara S, Edkins S, Teague J, Butler A, Dicks E, Stevens C, Tofts C, Avis T, Barthorpe S, Buck G, Cole J, Gray K, Halliday K, Harrison R, Hills K, Jenkinson A, Jones D, Menzies A, Mironenko T, Perry J, Raine K, Richardson D, Shepherd R, Small A, Varian J, West S, Widaa S, Mallya U, Moon J, Luo Y, Holder S, Smithson SF, Hurst JA, Clayton-Smith J, Kerr B, Boyle J, Shaw M, Vandeleur L, Rodriguez J, Slaugh R, Easton DF, Wooster R, Bobrow M, Srivastava AK, Stevenson RE, Schwar
American Journal of Human Genetics 2007 Feb; 80(2):345.
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Dioxin receptor is a ligand-dependent E3 ubiquitin ligase.
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