CUL4B polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western Blot analysis with CUL4B polyclonal antibody (Cat # PAB30584).
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Western Blot
Western Blot analysis with CUL4B polyclonal antibody (Cat # PAB30584).
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG sp
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum with CUL4B polyclonal antibody (Cat # PAB30584) shows strong nuclear positivity in Purkinje cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CUL4B polyclonal antibody (Cat # PAB30584) shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human CUL4B.
Immunogen
Recombinant protein corresponding to amino acids 9-152 of human CUL4B.
Sequence
GDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATS
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1 - 4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50 - 1:200)
Western Blot (1:100 - 1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis with CUL4B polyclonal antibody (Cat # PAB30584).
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Western Blot
Western Blot analysis with CUL4B polyclonal antibody (Cat # PAB30584).
Lane 1: Human cell line RT-4
Lane 2: Human cell line U-251MG spImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum with CUL4B polyclonal antibody (Cat # PAB30584) shows strong nuclear positivity in Purkinje cells.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with CUL4B polyclonal antibody (Cat # PAB30584) shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green. -
Gene Info — CUL4B
Entrez GeneID
8450Protein Accession#
Q13620Gene Name
CUL4B
Gene Alias
DKFZp686F1470, KIAA0695, MRXHF2, MRXSC, SFM2
Gene Description
cullin 4B
Gene Ontology
HyperlinkGene Summary
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023946|OTTHUMP00000023947|cullin-4B
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Interactome
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Pathway
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