LMNB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western Blot analysis of LMNB1 expression from rat brain tissue lyate with LMNB1 polyclonal antibody (Cat # PAB12282).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of LMNB1 on formalin fixed, paraffin embedded human mammary cancer with LMNB1 polyclonal antibody (Cat # PAB12282).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LMNB1.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human LMNB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
Different from the related rat and mouse sequence by three amino acids.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (0.1-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Immunohistochemistry (Frozen sections) (0.5-1 ug/mL)
Immunocytochemistry (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of LMNB1 expression from rat brain tissue lyate with LMNB1 polyclonal antibody (Cat # PAB12282).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of LMNB1 on formalin fixed, paraffin embedded human mammary cancer with LMNB1 polyclonal antibody (Cat # PAB12282).Immunohistochemistry (Frozen sections)
Immunocytochemistry
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Gene Info — LMNB1
Entrez GeneID
4001GeneBank Accession#
BC012295Protein Accession#
AAH12295Gene Name
LMNB1
Gene Alias
ADLD, LMN, LMN2, LMNB, MGC111419
Gene Description
lamin B1
Gene Ontology
HyperlinkGene Summary
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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A mitotic lamin B matrix induced by RanGTP required for spindle assembly.
Tsai MY, Wang S, Heidinger JM, Shumaker DK, Adam SA, Goldman RD, Zheng Y.
Science 2006 Mar; 311(5769):1887.
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In vitro posttranslational modification of lamin B cloned from a human T-cell line.
Pollard KM, Chan EK, Grant BJ, Sullivan KF, Tan EM, Glass CA.
Molecular and Cellular Biology 1990 May; 10(5):2164.
Application：IP, WB-Ce, Human, HeLa, Molt cells.
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A mitotic lamin B matrix induced by RanGTP required for spindle assembly.
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