MDM2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MDM2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S166 of human MDM2.
Sequence
AISET
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Specificity
MDM2 (Ab-166) Antibody detects endogenous levels of total MDM2 protein.
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 polyclonal antibody (Cat # PAB12253).Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using MDM2 polyclonal antibody (Cat # PAB12253, Red). -
Gene Info — MDM2
Entrez GeneID
4193Protein Accession#
Q00987Gene Name
MDM2
Gene Alias
HDMX, MGC71221, hdm2
Gene Description
Mdm2 p53 binding protein homolog (mouse)
Omim ID
164785Gene Ontology
HyperlinkGene Summary
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq
Other Designations
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|mouse double minute 2 homolog|p53-binding protein MDM2|ubiquitin-protein ligase E3 Mdm2
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Interactome
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Pathway
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Disease
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Publication Reference
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HER-2/neu induces p53 ubiquitination via Akt-mediated MDM2 phosphorylation.
Zhou BP, Liao Y, Xia W, Zou Y, Spohn B, Hung MC.
Nature Cell Biology 2001 Nov; 3(11):973.
Application:IF, IHC, IP, WB-Tr, Human, Mouse, Breast cancer, HEK 293T cells, MEFs, NIH/3T3 cells.
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A phosphatidylinositol 3-kinase/Akt pathway promotes translocation of Mdm2 from the cytoplasm to the nucleus.
Mayo LD, Donner DB.
PNAS 2001 Sep; 98(20):11598.
Application:IP, WB-Ce, WB-Re, Human, MCF-7 cells, Recombinant protein.
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p300/MDM2 complexes participate in MDM2-mediated p53 degradation.
Grossman SR, Perez M, Kung AL, Joseph M, Mansur C, Xiao ZX, Kumar S, Howley PM, Livingston DM.
Molecular Cell 1998 Oct; 2(4):405.
Application:IP-WB, IP, WB-Tr, Human, SAOS-2, B-JAB, U2OS cells.
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HER-2/neu induces p53 ubiquitination via Akt-mediated MDM2 phosphorylation.
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