Rabbit polyclonal antibody raised against partial recombinant SPTBN2.
Recombinant protein corresponding to human SPTBN2.
The epitope recognized by this antibody maps to a region between residues 2150 and 2200 of human spectrin, beta, non-erythrocytic 2 (spinocerebellar ataxia 5 ) using the numbering given in entry NP_008877.1
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Immunoprecipitation (2-5 ug/mg lysate) The optimal working dilution should be determined by the end user.
In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of SPTBN2 in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with SPTBN2 polyclonal antibody (Cat # PAB12023). Antibodies : Affinity purified SPTBN2 polyclonal antibody (Cat # PAB12023) used for IP at 3 ug/mg lysate. SPTBN2 was also immunoprecipitated by SPTBN2 polyclonal antibody (Cat # PAB12022), which recognizes a downstream epitope. Detection : Chemiluminescence with an exposure time of 30 seconds.