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Last updated: 2022/5/22
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ZEB1 polyclonal antibody 

  • Catalog # : PAB11610
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ZEB1.
  • Immunogen:
  • A synthetic peptide corresponding to human ZEB1.
  • Sequence:
  • C-RPQIRQKIENKPLQE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 122, 124
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Immunofluorescence (10 μg/mL)
    Western Blot (0.05-0.2 μg/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • ZEB1 polyclonal antibody (Cat # PAB11610) (0.05 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunofluorescence
  • Immunofluorescence
  • ZEB1 polyclonal antibody (Cat # PAB11610) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor 488 secondary antibody ( μg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 6935
  • Protein Accession#:
  • NP_001121600.1;NP_110378.3
  • Gene Name:
  • ZEB1
  • Gene Alias:
  • AREB6,BZP,DELTA-EF1,MGC133261,NIL-2-A,NIL-2A,NIL2A,TCF8,ZEB,ZFHEP,ZFHX1A
  • Gene Description:
  • zinc finger E-box binding homeobox 1
  • Gene Summary:
  • ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000019405,transcription factor 8 (represses interleukin 2 expression),zinc finger homeodomain enhancer-binding protein
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