ZEB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp with ZEB1 polyclonal antibody (Cat# PAB29343) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with ZEB1 polyclonal antibody (Cat# PAB29343) shows strong nuclear positivity in glial cells at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ZEB1 polyclonal antibody (Cat# PAB29343) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human ZEB1.
Immunogen
Recombinant protein corresponding to amino acids of human ZEB1.
Sequence
EAEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGST
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:200-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp with ZEB1 polyclonal antibody (Cat# PAB29343) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with ZEB1 polyclonal antibody (Cat# PAB29343) shows strong nuclear positivity in glial cells at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ZEB1 polyclonal antibody (Cat# PAB29343) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — ZEB1
Entrez GeneID
6935Protein Accession#
P37275Gene Name
ZEB1
Gene Alias
AREB6, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A
Gene Description
zinc finger E-box binding homeobox 1
Gene Ontology
HyperlinkGene Summary
ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM
Other Designations
OTTHUMP00000019405|transcription factor 8 (represses interleukin 2 expression)|zinc finger homeodomain enhancer-binding protein
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Interactome
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Disease
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