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Last updated: 2017/11/12

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RFP polyclonal antibody

  • Catalog # : PAB11376
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant RFP.
  • Immunogen:
  • Recombinant protein corresponding to full length mushroom polyp coral Discosoma RFP.
  • Host:
  • Rabbit
  • Specificity:
  • No reaction was observed against human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:160000)
    Western Blot (1:2000-1:10000)
    Immunohistochemistry (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot of RFP recombinant protein detected with RFP polyclonal antibody (Cat # PAB11376).
    Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract.
    Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band.
    A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with RFP polyclonal antibody (Cat # PAB11376) diluted 1:2,500 for 1 h at RT.
  • Immunohistochemistry
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
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