RFP polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot of RFP recombinant protein detected with RFP polyclonal antibody (Cat # PAB11376).
Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract.
Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band.
A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with RFP polyclonal antibody (Cat # PAB11376) diluted 1:2,500 for 1 h at RT.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant RFP.
Immunogen
Recombinant protein corresponding to full length mushroom polyp coral Discosoma RFP.
Host
Rabbit
Specificity
No reaction was observed against human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:160000)
Western Blot (1:2000-1:10000)
Immunohistochemistry (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot of RFP recombinant protein detected with RFP polyclonal antibody (Cat # PAB11376).
Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract.
Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kDa band.
A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with RFP polyclonal antibody (Cat # PAB11376) diluted 1:2,500 for 1 h at RT.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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