Product Browser

Last updated: 2017/11/12
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

RAD9 (phospho S1129) polyclonal antibody

  • Catalog # : PAB11309
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAD9.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S1129 of Saccharomyces cerevisiae RAD9.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • This phospho specific polyclonal antibody reacts with phosphorylated pS1260 of yeast Rad9. Reactivity with non-phosphorylated yeast Rad9 is minimal by ELISA and immunoblotting. No reactivity is expected against the human or mouse analogs of RAD9. Cross reactivity may occur with auto-phosphorylated Rad53 kinase.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Sandwich ELISA (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Immunoblotting of RAD9 (phospho S1129) polyclonal antibody (Cat # PAB11309) was used at a 1 : 200 dilution incubated 8 h at room temperature to detect RAD9 by Western blot.
    Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae RAD9 (aa 991-1309 ~60 KDa) on a 4-20% Criterion gel for SDS-PAGE as follows :
    Lane 1 - non-phosphorylated wild type yeast RAD9, Lane 2 - in vitro phosphorylated wild type yeast RAD9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant RAD9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.
    Phosphorylation of RAD9 was by treatment with ATP and RAD53 kinase.
    Detection occurred using a 1:5,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Locus:11q13.1-q13.2;OMIM603761;GDB5592334;SwissProtQ99638
  • Gene Name:
  • RAD9
  • Gene Alias:
  • -
  • Gene Description:
  • DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate
  • Other Designations:
  • Rad9p
  • RSS
  • YouTube
  • Linkedin
  • Facebook