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Last updated: 2017/10/15
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SPRY4 polyclonal antibody

  • Catalog # : PAB11269
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SPRY4.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 306-322 of human SPRY4.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • Expect reactivity only with the SPRY4A splice variant of this protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:3000-1:12000)
    Western Blot (1:100-1:1000)
    Immunohistochemistry (1-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using SPRY4 polyclonal antibody (Cat # PAB11269) shows detection of a doublet band ~35 kDa corresponding to human SPRY4.
    Approximately 30 ug of HeLa (lane 1), SW-13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE.
    The primary antibody diluted to 1:100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of SPRY4 polyclonal antibody (Cat # PAB11269) was used at a 2.5 ug/mL to detect cytoplasmic signal with moderate to strong staining and low background in a variety of tissues including heart, prostate, intestine, kidney and pancreas.
    Strong specific staining was noted at particular cell types in some tissues (i.e. Sertoli and Leydig cells).
    The antibody shows very good signal to background at 2.5 ug/mL.
    This image shows SPRY4 staining of human heart tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of SPRY4 polyclonal antibody (Cat # PAB11269) shows strong cytoplasmic and membranous staining of tumor cells in human liver tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Brown color indicates presence of protein, blue color shows cell nuclei.
    Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q9C004;NP_112226
  • Gene Name:
  • SPRY4
  • Gene Alias:
  • -
  • Gene Description:
  • sprouty homolog 4 (Drosophila)
  • Gene Summary:
  • SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM
  • Other Designations:
  • sprouty homolog 4
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