Western blot using SPRY4 polyclonal antibody (Cat # PAB11269) shows detection of a doublet band ~35 kDa corresponding to human SPRY4. Approximately 30 ug of HeLa (lane 1), SW-13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE. The primary antibody diluted to 1:100.
Immunohistochemistry of SPRY4 polyclonal antibody (Cat # PAB11269) was used at a 2.5 ug/mL to detect cytoplasmic signal with moderate to strong staining and low background in a variety of tissues including heart, prostate, intestine, kidney and pancreas. Strong specific staining was noted at particular cell types in some tissues (i.e. Sertoli and Leydig cells). The antibody shows very good signal to background at 2.5 ug/mL. This image shows SPRY4 staining of human heart tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
Immunohistochemistry of SPRY4 polyclonal antibody (Cat # PAB11269) shows strong cytoplasmic and membranous staining of tumor cells in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM