SPRY4 recombinant monoclonal antibody, clone R01-6K5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1: MCF-7, Lane2: 3T3 and Lane3: C6 lysates with SPRY4 recombinant monoclonal antibody, clone R01-6K5 (Cat # RAB01897).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SPRY4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SPRY4.
Theoretical MW (kDa)
Calculated MW: 33 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1: MCF-7, Lane2: 3T3 and Lane3: C6 lysates with SPRY4 recombinant monoclonal antibody, clone R01-6K5 (Cat # RAB01897).Immunohistochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — SPRY4
Entrez GeneID
81848Protein Accession#
Q9C004Gene Name
SPRY4
Gene Alias
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Gene Description
sprouty homolog 4 (Drosophila)
Omim ID
607984Gene Ontology
HyperlinkGene Summary
SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM
Other Designations
sprouty homolog 4
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Interactome
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Pathway
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Disease
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