Immunoblot using DIABLO polyclonal antibody (Cat # PAB11261) detects a 26 kDa band when 1 ug of recombinant DIABLO is applied (lane 1). Lane 2 shows DIABLO detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 micro;M etoposide. Recombinant DIABLO migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of DIABLO polyclonal antibody.
Western Blot (Recombinant protein)
DIABLO polyclonal antibody (Cat # PAB11261) is shown to detect a 25-26 kDa band in partially purified recombinant human DIABLO protein by immunoblot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1 : 1000 dilution of DIABLO polyclonal antibody in TBST. Detection occurs using a 1 : 1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'.
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq
0610041G12Rik,diablo,direct IAP-binding protein with low pI,mitochondrial Smac protein,second mitochondria-derived activator of caspase