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Last updated: 2016/12/4
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DIABLO polyclonal antibody

  • Catalog # : PAB11261
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant DIABLO.
  • Immunogen:
  • Recombinant His fusion protein corresponding to amino acids 56-239 of human DIABLO.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblot using DIABLO polyclonal antibody (Cat # PAB11261) detects a 26 kDa band when 1 ug of recombinant DIABLO is applied (lane 1).
    Lane 2 shows DIABLO detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied.
    Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 micro;M etoposide.
    Recombinant DIABLO migrates slower than the native form because of the His6-tag.
    The blot was incubated overnight with a 1:1000 dilution of DIABLO polyclonal antibody.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • DIABLO polyclonal antibody (Cat # PAB11261) is shown to detect a 25-26 kDa band in partially purified recombinant human DIABLO protein by immunoblot.
    Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively.
    The blot was incubated overnight with a 1 : 1000 dilution of DIABLO polyclonal antibody in TBST.
    Detection occurs using a 1 : 1000 dilution of HRP Goat-a-Rabbit with visualization via ECL.
    Film exposure approximately 1'.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • DIABLO
  • Gene Alias:
  • DIABLO-S,FLJ10537,FLJ25049,SMAC,SMAC3
  • Gene Description:
  • diablo homolog (Drosophila)
  • Gene Summary:
  • This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq
  • Other Designations:
  • 0610041G12Rik,diablo,direct IAP-binding protein with low pI,mitochondrial Smac protein,second mitochondria-derived activator of caspase
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