RAB11FIP3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using RAB11FIP3 polyclonal antibody (Cat # PAB11253) to detect RAB11FIP3 (arrowhead) in NIH/3T3 cells (lane 1), HeLa cells (lane 2) and 293T cells (lane 3).
The primary antibody at a 1 : 2,000 dilution.
Personal Communication, P. Randazzo, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant RAB11FIP3.
Immunogen
Recombinant protein corresponding to C-terminus one-third of human RAB11FIP3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:1000-1:3000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using RAB11FIP3 polyclonal antibody (Cat # PAB11253) to detect RAB11FIP3 (arrowhead) in NIH/3T3 cells (lane 1), HeLa cells (lane 2) and 293T cells (lane 3).
The primary antibody at a 1 : 2,000 dilution.
Personal Communication, P. Randazzo, CCR-NCI, Bethesda, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAB11FIP3
Entrez GeneID
9727Protein Accession#
NP_055515;O75154Gene Name
RAB11FIP3
Gene Alias
KIAA0665, Rab11-FIP3
Gene Description
RAB11 family interacting protein 3 (class II)
Omim ID
608738Gene Ontology
HyperlinkGene Summary
Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP3 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]).[supplied by OMIM
Other Designations
PAC196A12.1|eferin|rab11-family interacting protein 3
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Interactome
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Pathway
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Publication Reference
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Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1.
Shiba T, Koga H, Shin HW, Kawasaki M, Kato R, Nakayama K, Wakatsuki S.
PNAS 2006 Oct; 103(42):15416.
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Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis.
Fielding AB, Schonteich E, Matheson J, Wilson G, Yu X, Hickson GR, Srivastava S, Baldwin SA, Prekeris R, Gould GW.
The EMBO Journal 2005 Oct; 24(19):3389.
Application：IF, WB, Human, Mouse, CHO, HeLa cells, Recombinant protein.
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Identification of a novel Rab11/25 binding domain present in Eferin and Rip proteins.
Prekeris R, Davies JM, Scheller RH.
The Journal of Biological Chemistry 2001 Oct; 276(42):38966.
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Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1.
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