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Last updated: 2018/1/14
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His tag polyclonal antibody

  • Catalog # : PAB10339
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptides of His tag.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to His tag.
  • Sequence:
  • Host:
  • Rabbit
  • Specificity:
  • In western blotting of bacterial extracts, This antibody does not cross-react with endogenous proteins.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:1000-1:3000)
    Immunohistochemistry (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • His tag polyclonal antibody (Cat # PAB10339) detects His-tagged recombinant proteins by western blot.
    The blot was blocked with 3% BSA in TBST for 45 min at RT.
    Antibody was incubated with blot at a 1 : 1,000 dilution in TBST with 3% BSA for 1 hour at RT.
    Detection occurred using HRP Gt-a-Rabbit IgG diluted 1 : 80,000 in blocking buffer for 30 min at RT.
    Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~ 42 KDa).
    Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~ 40 KDa).
    A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.
    Proteins were transferred to nitrocellulose using standard methods.
    Molecular weights were estimated by comparison to standards (Lane M).
  • Immunohistochemistry
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry
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