His tag polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
His tag polyclonal antibody (Cat # PAB10339) detects His-tagged recombinant proteins by western blot.
The blot was blocked with 3% BSA in TBST for 45 min at RT.
Antibody was incubated with blot at a 1 : 1,000 dilution in TBST with 3% BSA for 1 hour at RT.
Detection occurred using HRP Gt-a-Rabbit IgG diluted 1 : 80,000 in blocking buffer for 30 min at RT.
Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~ 42 KDa).
Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~ 40 KDa).
A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.
Proteins were transferred to nitrocellulose using standard methods.
Molecular weights were estimated by comparison to standards (Lane M).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptides of His tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Sequence
HHHHHH
Host
Rabbit
Specificity
In western blotting of bacterial extracts, This antibody does not cross-react with endogenous proteins.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:1000-1:3000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
His tag polyclonal antibody (Cat # PAB10339) detects His-tagged recombinant proteins by western blot.
The blot was blocked with 3% BSA in TBST for 45 min at RT.
Antibody was incubated with blot at a 1 : 1,000 dilution in TBST with 3% BSA for 1 hour at RT.
Detection occurred using HRP Gt-a-Rabbit IgG diluted 1 : 80,000 in blocking buffer for 30 min at RT.
Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~ 42 KDa).
Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~ 40 KDa).
A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.
Proteins were transferred to nitrocellulose using standard methods.
Molecular weights were estimated by comparison to standards (Lane M).Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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