Western blot using RSL1D1 polyclonal antibody (Cat # PAB10306) shows detection of over-expressed RSL1D1 in lysates from HeLa cells transfected with Flag-RSL1D1. Lanes 1 and 3 contain lysate from Flag-RSL1D1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1 : 500 dilution of RSL1D1 polyclonal antibody. The band at 75 KDa, indicated by the arrowhead, corresponds to RSL1D1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
Immunofluorescence microscopy of HeLa cells transfected with RSL1D1 polyclonal antibody (Cat # PAB10306) . In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.