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Last updated: 2017/5/21
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RSL1D1 polyclonal antibody

  • Catalog # : PAB10306
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RSL1D1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human RSL1D1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is specific to human PBK1 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2500-1:10000)
    Western Blot (1:500-1:2000)
    Immunoprecipitation (1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using RSL1D1 polyclonal antibody (Cat # PAB10306) shows detection of over-expressed RSL1D1 in lysates from HeLa cells transfected with Flag-RSL1D1.
    Lanes 1 and 3 contain lysate from Flag-RSL1D1 transfected HeLa cells.
    Lanes 2 and 4 contain lysate from cells transfected with null vector.
    Lanes 1 and 2 were blotted with anti-Flag antibody.
    Lanes 3 and 4 were probed with a 1 : 500 dilution of RSL1D1 polyclonal antibody.
    The band at 75 KDa, indicated by the arrowhead, corresponds to RSL1D1.
    Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopy of HeLa cells transfected with RSL1D1 polyclonal antibody (Cat # PAB10306) .
    In the overlay, specific antibody staining is shown to co-localize with recombinant protein.
    Cells were fixed with methanol prior to staining.
    Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • RSL1D1
  • Gene Alias:
  • CSIG,DKFZP564M182,L12,MGC138433,MGC142259,PBK1
  • Gene Description:
  • ribosomal L1 domain containing 1
  • Other Designations:
  • cellular senescence inhibited gene protein
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