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Last updated: 2017/8/20
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POU5F1 polyclonal antibody

  • Catalog # : PAB10296
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human POU5F1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Dog, Horse, Human, Macaque, Mink, Mouse, Pig
  • Specificity:
  • This antibody reacts with human and mouse Oct-4 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:350000)
    Western Blot (1:1000-1:1500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using POU5F1 polyclonal antibody (Cat # PAB10296) shows detection of endogenous POU5F1 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2).
    The band at ~39 kDa (arrowhead) corresponds to POU5F1.
    After transfer, the membrane was blocked with 5% BSA.
    Primary antibody was used at a 1 : 1,000 dilution in PBS containing 5% BSA.
    The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with POU5F1 (lanes 3 and 4, respectively).
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5460
  • Protein Accession#:
  • NP_976034;Q01860
  • Gene Name:
  • POU5F1
  • Gene Alias:
  • MGC22487,OCT3,OCT4,OTF3,OTF4
  • Gene Description:
  • POU class 5 homeobox 1
  • Other Designations:
  • OTTHUMP00000029292,POU domain, class 5, transcription factor 1,POU-type homeodomain-containing DNA-binding protein,octamer-binding transcription factor-3
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