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Last updated: 2017/11/19
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NOTCH1 polyclonal antibody

  • Catalog # : PAB10291
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of NOTCH1.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus the cleaved N intracellular domain (NICD) of human NOTCH1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antiserum is directed against human NOTCH 1. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Sandwich ELISA (1:20000-1:60000)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Immunoblotting of NOTCH1 polyclonal antibody (Cat # PAB10291) was used at a 1 : 500 dilution to detect mouse NOTCH1 by Western blot.
    Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods.
    A reaction with diluted primary antibody was followed by washing, reaction with a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development.
    Lane 1 : No transfection.
    Lane 2 : N1 (mouse deleted extracellular domain)-myc.
    Lane 3 : N1 (mouse intracellular domain)-myc.
    Lane 4 : N2 (mouse deleted extracellular domain)-myc.
    Lane 5 : N2 (mouse intracellular domain)-myc.
    Lane 6 : N3 (mouse deleted extracellular domain)-myc.
    Lane 7 : N3 (mouse intracellular domain)-myc.
    Lane 8 : N4 (mouse deleted extracellular domain)-myc.
    Lane 9 : N4 (mouse intracellular domain)-myc.
    Lane 10 : N1 (mouse deleted extracellular doma
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4851
  • Gene Name:
  • NOTCH1
  • Gene Alias:
  • TAN1,hN1
  • Gene Description:
  • Notch homolog 1, translocation-associated (Drosophila)
  • Gene Summary:
  • This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022594,neurogenic locus notch homolog protein 1,notch1,translocation-associated notch protein TAN-1
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