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Last updated: 2016/12/4
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REL polyclonal antibody

  • Catalog # : PAB10284
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of REL.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus human REL.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:1000)
    Gel Supershift Assay (0.5-1.0 ul per assay)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblot of HeLa cell extract.
    All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
    The membrane was blocked in 5% dry milk for 2 h.
    After washing, a 1 : 1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.
    Washes with buffer were performed 4 times for 5' each.
    The immunoblot was incubated with secondary antibody (HRP Goat-a-RabbitIgG [H&L]) diluted 1:2,000 for 1h.
    Washes with TBS precededcolor development.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5966
  • Gene Name:
  • REL
  • Gene Alias:
  • C-Rel
  • Gene Description:
  • v-rel reticuloendotheliosis viral oncogene homolog (avian)
  • Gene Summary:
  • The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM
  • Other Designations:
  • C-Rel proto-oncogene protein,oncogene REL, avian reticuloendotheliosis,v-rel avian reticuloendotheliosis viral oncogene homolog,v-rel reticuloendotheliosis viral oncogene homolog
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