REL polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Immunoblot of HeLa cell extract.
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
The membrane was blocked in 5% dry milk for 2 h.
After washing, a 1 : 1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.
Washes with buffer were performed 4 times for 5' each.
The immunoblot was incubated with secondary antibody (HRP Goat-a-RabbitIgG [H&L]) diluted 1:2,000 for 1h.
Washes with TBS precededcolor development.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of REL.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus human REL.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
Gel Supershift Assay (0.5-1.0 ul per assay)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblot of HeLa cell extract.
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
The membrane was blocked in 5% dry milk for 2 h.
After washing, a 1 : 1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.
Washes with buffer were performed 4 times for 5' each.
The immunoblot was incubated with secondary antibody (HRP Goat-a-RabbitIgG [H&L]) diluted 1:2,000 for 1h.
Washes with TBS precededcolor development.Enzyme-linked Immunoabsorbent Assay
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Gene Info — REL
Entrez GeneID
5966Gene Name
REL
Gene Alias
C-Rel
Gene Description
v-rel reticuloendotheliosis viral oncogene homolog (avian)
Omim ID
164910Gene Ontology
HyperlinkGene Summary
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM
Other Designations
C-Rel proto-oncogene protein|oncogene REL, avian reticuloendotheliosis|v-rel avian reticuloendotheliosis viral oncogene homolog|v-rel reticuloendotheliosis viral oncogene homolog
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Interactome
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Disease
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