Western blot analysis is shown using RAD52 polyclonal antibody (Cat # PAB10230) to detect human RAD52 present in a HeLa nuclear extract (panel B). Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~ 63.0 KDa is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 5,000 for 45 min.
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq
DNA repair protein RAD52,RAD52 homolog,recombination protein RAD52,rhabdomyosarcoma antigen MU-RMS-40.23