RAD52 monoclonal antibody, clone 5E11E7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: MCF-7 cell; Lane 3: MCF-7 cell; Lane 4: C6 cell with RAD52 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD52-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with RAD52 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD52 monoclonal antibody, clone 5E11E7.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with RAD52 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAD52.
Immunogen
Recombinant protein corresponding to amino acid 269-418 of human RAD52 from E. coli.
Host
Mouse
Theoretical MW (kDa)
46.2kDa
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell; Lane 2: MCF-7 cell; Lane 3: MCF-7 cell; Lane 4: C6 cell with RAD52 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD52-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with RAD52 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD52 monoclonal antibody, clone 5E11E7.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with RAD52 monoclonal antibody (green) and negative control (red). -
Gene Info — RAD52
Entrez GeneID
5893Gene Name
RAD52
Gene Alias
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Gene Description
RAD52 homolog (S. cerevisiae)
Omim ID
600392Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq
Other Designations
DNA repair protein RAD52|RAD52 homolog|recombination protein RAD52|rhabdomyosarcoma antigen MU-RMS-40.23
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Interactome
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Pathway
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Disease
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