RAD23B polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using RAD23B polyclonal antibody (Cat # PAB10058) shows detection of a band at ~58 KDa (arrowhead) corresponding to RAD23B present in a HeLa whole cell lysate.
Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data notshown).
Approximately 33 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS.
The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature (800 nmchannel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red).
IRDye™800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR,Inc.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RAD23B.
Immunogen
A synthetic peptide corresponding to amino acids 163-176 of human RAD23B.
Host
Goat
Reactivity
Chimpanzee, Dog, Human, Mouse, Rat, Yeast
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:16000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using RAD23B polyclonal antibody (Cat # PAB10058) shows detection of a band at ~58 KDa (arrowhead) corresponding to RAD23B present in a HeLa whole cell lysate.
Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data notshown).
Approximately 33 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS.
The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature (800 nmchannel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red).
IRDye™800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR,Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAD23B
Entrez GeneID
5887Protein Accession#
P54727;NP_002865Gene Name
RAD23B
Gene Alias
HHR23B, HR23B, P58
Gene Description
RAD23 homolog B (S. cerevisiae)
Omim ID
600062Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq
Other Designations
OTTHUMP00000021857|RAD23, yeast homolog of, B|UV excision repair protein RAD23 homolog B|XP-C repair complementing complex 58 kDa|XP-C repair complementing protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of a novel RAD23B mRNA splice variant in the human testis.
Huang X, Wang H, Xu M, Lu L, Xu Z, Li J, Zhou Z, Sha J.
Journal of Andrology 2004 May; 25(3):363.
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Involvement of the DNA repair protein hHR23 in p53 degradation.
Glockzin S, Ogi FX, Hengstermann A, Scheffner M, Blattner C.
Molecular and Cellular Biology 2003 Dec; 23(24):8960.
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Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B.
Fujiwara K, Tenno T, Sugasawa K, Jee JG, Ohki I, Kojima C, Tochio H, Hiroaki H, Hanaoka F, Shirakawa M.
The Journal of Biological Chemistry 2004 Feb; 279(6):4760.
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Expression of a novel RAD23B mRNA splice variant in the human testis.
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