Western blot using RAD23B polyclonal antibody (Cat # PAB10058) shows detection of a band at ~58 KDa (arrowhead) corresponding to RAD23B present in a HeLa whole cell lysate. Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data notshown). Approximately 33 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature (800 nmchannel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye™800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR,Inc.
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq
OTTHUMP00000021857,RAD23, yeast homolog of, B,UV excision repair protein RAD23 homolog B,XP-C repair complementing complex 58 kDa,XP-C repair complementing protein