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Last updated: 2016/12/4
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BACH1 polyclonal antibody

  • Catalog # : PAB10027
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant BACH1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 92-104 of human BACH1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Human
  • Specificity:
  • Expect reactivity with isoform 1 and isoform 2 of BACH1.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:10000-1:44000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using BACH1 polyclonal antibody (Cat # PAB10027) shows detection of a band at~105 KDa (Lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead).
    Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction.
    Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:1,000.
    Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
    IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 571
  • Protein Accession#:
  • Q9BX63;NP_114432
  • Gene Name:
  • BACH1
  • Gene Alias:
  • -
  • Gene Description:
  • BTB and CNC homology 1, basic leucine zipper transcription factor 1
  • Gene Summary:
  • This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • BTB and CNC homology 1,OTTHUMP00000096564
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