BACH1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of (at 1:500) Lane 1: Mouse lung tissue lysate, Lane 2: AML12 cell lysate, Lane 3: PMVEC cell lysate, Lane 4: 293T cell lysate, Lane 5: Hela cell lysate, Lane 6: SH-SY5Y cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analyzes of human tonsil carcinoma tissue (at 1:50) showing Nucleus staining. Negative control (the below) using PBS instead of primary antibody, secondary antibody is goat anti-rabbit IgG-biotin followed by avidin-peroxidase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human BACH1.
Immunogen
A synthetic peptide corresponding to amino acids 131-180 of human BACH1.
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human
Specificity
BACH1 polyclonal antibody detects endogenous levels of BACH1 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Isotype
IgG
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of (at 1:500) Lane 1: Mouse lung tissue lysate, Lane 2: AML12 cell lysate, Lane 3: PMVEC cell lysate, Lane 4: 293T cell lysate, Lane 5: Hela cell lysate, Lane 6: SH-SY5Y cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analyzes of human tonsil carcinoma tissue (at 1:50) showing Nucleus staining. Negative control (the below) using PBS instead of primary antibody, secondary antibody is goat anti-rabbit IgG-biotin followed by avidin-peroxidase.Immunofluorescence
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Gene Info — BACH1
Entrez GeneID
571Protein Accession#
O14867Gene Name
BACH1
Gene Alias
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Gene Description
BTB and CNC homology 1, basic leucine zipper transcription factor 1
Omim ID
602751Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq
Other Designations
BTB and CNC homology 1|OTTHUMP00000096564
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Interactome
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Disease
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