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Last updated: 2016/12/4
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EIF3F polyclonal antibody

  • Catalog # : PAB10008
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EIF3F.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 114-125 of human EIF3F.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Fruit fly, Human, Mouse, Pufferfishes, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using EIF3F polyclonal antibody (Cat # PAB10008) shows detection of endogenous EIF3F in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2).
    Cdk11 is responsible for phosphorylating EIF3F in vivo.
    After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 200.
    This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.
    Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 8665
  • Gene Name:
  • EIF3F
  • Gene Alias:
  • EIF3S5,eIF3-p47
  • Gene Description:
  • eukaryotic translation initiation factor 3, subunit F
  • Other Designations:
  • eIF3-epsilon,eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD),eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
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