EIF3F polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using EIF3F polyclonal antibody (Cat # PAB10008) shows detection of endogenous EIF3F in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2).
Cdk11 is responsible for phosphorylating EIF3F in vivo.
After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 200.
This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.
Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF3F.
Immunogen
A synthetic peptide corresponding to amino acids 114-125 of human EIF3F.
Host
Rabbit
Reactivity
Bovine, Chicken, Chimpanzee, Fruit fly, Human, Mouse, Pufferfishes, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using EIF3F polyclonal antibody (Cat # PAB10008) shows detection of endogenous EIF3F in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2).
Cdk11 is responsible for phosphorylating EIF3F in vivo.
After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 200.
This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.
Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF3F
Entrez GeneID
8665Protein Accession#
O00303Gene Name
EIF3F
Gene Alias
EIF3S5, eIF3-p47
Gene Description
eukaryotic translation initiation factor 3, subunit F
Omim ID
603914Gene Ontology
HyperlinkGene Summary
O
Other Designations
eIF3-epsilon|eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD)|eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa
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Interactome
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Publication Reference
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Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly.
Asano K, Vornlocher HP, Richter-Cook NJ, Merrick WC, Hinnebusch AG, Hershey JW.
The Journal of Biological Chemistry 1997 Oct; 272(43):27042.
Application：WB-Ce, Human, HeLa cells.
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Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly.
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