Ngf polyclonal antibody
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Specification
Product Description
Sheep polyclonal antibody raised against native Ngf.
Immunogen
Native mouse beta NGF purified from submaxillary salivary gland.
Host
Sheep
Reactivity
Birds, Human, Mouse, Rat
Specificity
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Form
Lyophilized
Purification
Protein G purification
Purity
95% by PAGE
Isotype
IgG
Recommend Usage
1-site ELISA (1-3 ug/mL)
Immunohistochemistry (1-3 ug/mL)
inhibition of biological activity (1-3 ug/mL in vitro)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from antiserum.
Storage Instruction
Store lyophilized material at 2-8°C or -20°C.
After reconstitution with 500 uL of sterile water, store and keep aliquots at -20°C for up to six months for a higher stability, and at 2-8°C with an appropriate antibacterial agent for up to one week. Centrifuge to remove any insoluble material. Glycerol (1:1) may be added for an additional stability.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Inhibition Assay
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Gene Info — Ngf
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