Ngf polyclonal antibody
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Specification
Product Description
Sheep polyclonal antibody raised against native Ngf.
Immunogen
Native purified mouse Ngf from submaxillary salivary gland.
Host
Sheep
Reactivity
Birds, Human, Mouse, Rat
Specificity
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Form
Lyophilized
Purity
Whole serum
Recommend Usage
ELISA (1:15000)
Immunohistochemistry (1:1000-1:5000)
Inhibition assay (1:10-50 for in vitro 5-10 uL/g body weight for in vivo)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS.
Storage Instruction
Store at -20°C.
After reconstitution with 250 uL of sterile water, store at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
Inhibition Assay
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Gene Info — Ngf
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