E2F1 (phospho S337) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The E2F1 (phospho S337) polyclonal antibody (Cat # PAB0449) is used in Western blot to detect Phospho-E2F1-S337 in T-47D (left), HepG2 (middle), and A2058 (right) cell lysates
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with E2F1 (phospho S337) polyclonal antibody (Cat # PAB0449) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Product Citations :
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of E2F1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S337 of human E2F1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The E2F1 (phospho S337) polyclonal antibody (Cat # PAB0449) is used in Western blot to detect Phospho-E2F1-S337 in T-47D (left), HepG2 (middle), and A2058 (right) cell lysatesImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with E2F1 (phospho S337) polyclonal antibody (Cat # PAB0449) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Product Citations : -
Gene Info — E2F1
Entrez GeneID
1869Protein Accession#
NP_005216;Q01094Gene Name
E2F1
Gene Alias
E2F-1, RBAP1, RBBP3, RBP3
Gene Description
E2F transcription factor 1
Omim ID
189971Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq
Other Designations
OTTHUMP00000030661|retinoblastoma-associated protein 1
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Interactome
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Publication Reference
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c-Myc-regulated microRNAs modulate E2F1 expression.
O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT.
Nature 2005 Jun; 435(7043):839.
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Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors.
Joshi B, Ordonez-Ercan D, Dasgupta P, Chellappan S.
Oncogene 2005 Mar; 24(13):2204.
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Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism.
Wang C, Hou X, Mohapatra S, Ma Y, Cress WD, Pledger WJ, Chen J.
The Journal of Biological Chemistry 2005 Feb; 280(13):12339.
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c-Myc-regulated microRNAs modulate E2F1 expression.
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