Products

Western Blot (Cell lysate)

Western blot analysis of E2F1 expression in Lane 1: Molt4, Lane 2: C6, Lane 3: NIH3T3 whole cell lysates with E2F1 recombinant monoclonal antibody (Cat # RAB02490).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using E2F1 recombinant monoclonal antibody (Cat # RAB02490). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.120). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry

Immunofluorescence

Immunofluorescent analysis of HeLa cells with E2F1 recombinant monoclonal antibody (Cat # RAB02490). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Immunoprecipitation

Entrez GeneID

1869

Protein Accession#

Q01094

Gene Name

E2F1

Gene Alias

E2F-1, RBAP1, RBBP3, RBP3

Gene Description

E2F transcription factor 1

Omim ID

189971

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq

Other Designations

OTTHUMP00000030661|retinoblastoma-associated protein 1