BAG3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of BAG3. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. Tissue lysates, normalized for total protein (20 ug/lane) , were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
Immunohistochemistry (Frozen sections)
Frozen mouse muscle tissue section stained for BAG3 expression using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant BAG3.
Immunogen
Recombinant protein corresponding to C-terminus 196 amino acids of human BAG3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of BAG3. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. Tissue lysates, normalized for total protein (20 ug/lane) , were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.Immunohistochemistry (Frozen sections)
Frozen mouse muscle tissue section stained for BAG3 expression using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins.Immunoprecipitation
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Gene Info — BAG3
Entrez GeneID
9531Gene Name
BAG3
Gene Alias
BAG-3, BIS, CAIR-1, MGC104307
Gene Description
BCL2-associated athanogene 3
Omim ID
603883Gene Ontology
HyperlinkGene Summary
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq
Other Designations
BAG-family molecular chaperone regulator-3|BCL2-binding athanogene 3|Bcl-2-binding protein|OTTHUMP00000020599|docking protein CAIR-1
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Interactome
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Disease
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Publication Reference
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BAG3 is a negative regulator of ciliogenesis in glioblastoma and triple-negative breast cancer cells.
Benedikt Linder, Caterina Klein, Marina E Hoffmann, Florian Bonn, Ivan Dikic, Donat Kögel.
Journal of Cellular Biochemistry 2022 Jan; 123(1):77.
Application：WB-Tr, Human, BT-549, U251, U343 cells.
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BAG3 Overexpression and Cytoprotective Autophagy Mediate Apoptosis Resistance in Chemoresistant Breast Cancer Cells.
Das CK, Linder B, Bonn F, Rothweiler F, Dikic I, Michaelis M, Cinatl J, Mandal M, Kogel D.
Neoplasia 2018 Mar; 20:263.
Application：WB, Human, BT-549, MDA-MB-468 cells.
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Familial Dilated Cardiomyopathy Caused by a Novel Frameshift in the BAG3 Gene.
Toro R, Perez-Serra A, Campuzano O, Moncayo-Arlandi J, Allegue C, Iglesias A, Mangas A, Brugada R.
PLoS One 2016 Jul; 11(7):e0158730.
Application：WB-Ti, Human, Heart.
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Modulation of BAG3 Expression and Proteasomal Activity by sAPPα Does Not Require Membrane-Tethered Holo-APP.
Kundu A, Milosch N, Antonietti P, Baumkotter F, Zymny A, Muller UC, Kins S, Hajieva P, Behl C, Kogel D.
Molecular Neurobiology 2016 Nov; 53(9):5985.
Application：WB, Human, SH-SY5Y cells.
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The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.
Liao Q, Ozawa F, Friess H, Zimmermann A, Takayama S, Reed JC, Kleeff J, Buchler MW.
FEBS Letters 2001 Aug; 503(2-3):151.
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BAG3 is a negative regulator of ciliogenesis in glioblastoma and triple-negative breast cancer cells.
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