Western blot analysis of BAG3. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. Tissue lysates, normalized for total protein (20 ug/lane) , were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
Immunohistochemistry (Frozen sections)
Frozen mouse muscle tissue section stained for BAG3 expression using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins.
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq
BAG-family molecular chaperone regulator-3,BCL2-binding athanogene 3,Bcl-2-binding protein,OTTHUMP00000020599,docking protein CAIR-1