TFF1 (Human) Recombinant Protein
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Specification
Product Description
Human TFF1 recombinant proteind expressed in Escherichia coli..
Sequence
EAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF.
Host
Escherichia coli
Theoretical MW (kDa)
13.2
Specificity
TFF1 Human
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 97% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 10 ug/mL, measured by a chemotaxis bioassay using human MCF-7 cells, corresponding to a specific activity > 100 IU/mg.
Storage Buffer
Lyophilized from a solution containing 20 mM PB, pH 7.4, 150 mM NaCl. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — TFF1
Entrez GeneID
7031Protein Accession#
P04155Gene Name
TFF1
Gene Alias
BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2
Gene Description
trefoil factor 1
Omim ID
113710Gene Ontology
HyperlinkGene Summary
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq
Other Designations
breast cancer estrogen-inducible sequence|gastrointestinal trefoil protein pS2
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