CD28 (Human) Recombinant Protein
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Specifications
Product Description
Human CD28 (P10747, 19 a.a. - 152 a.a.) partial length recombinant protein with hIgG-His tag expressed in Baculovirus expression system.
Sequence
NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP
Host
Viruses
Theoretical MW (kDa)
42.4
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
ED50 is < 10 ug/mL, measured by the binding ability in a functional ELISA with Human CD80.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In Phosphate-Buffer Saline pH 7.4 (10% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CD28
Entrez GeneID
940Protein Accession#
P10747Gene Name
CD28
Gene Alias
MGC138290, Tp44
Gene Description
CD28 molecule
Omim ID
186760Gene Ontology
HyperlinkGene Summary
CD28 costimulation is essential for CD4 (MIM 186940)-positive T-cell proliferation, survival, interleukin-2 (IL2; MIM 147680) production, and T-helper type-2 (Th2) development.[supplied by OMIM
Other Designations
CD28 antigen|CD28 antigen (Tp44)|T-cell-specific surface glycoprotein
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Interactomes
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Pathways
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Diseases
- Acute Disease
- Arthritis
- Asthma
- Behcet Syndrome
- Bronchiolitis
- Carcinoma
- Celiac Disease
- Cholangitis
- Colon cancer
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