CD28 (Human) Recombinant Protein
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Specification
Product Description
Human CD28 (NP_006130.1, 19 a.a. - 152 a.a.) partial recombinant protein with a 6xHis tag at the C-terminus expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 97% (determined by SDS-PAGE)
Endotoxin Level
< 0.1 EU/ug of protein (determined by LAL method)
Activity
Measured by the binding ability in a functional ELISA. Immobilized recombinant human CD28 at 10 ug/mL (100 uL/well) can bind biotinylated human CD86 with a linear range of 3-12 ug/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Storage Instruction
Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — CD28
Entrez GeneID
940Protein Accession#
P10747Gene Name
CD28
Gene Alias
MGC138290, Tp44
Gene Description
CD28 molecule
Omim ID
186760Gene Ontology
HyperlinkGene Summary
CD28 costimulation is essential for CD4 (MIM 186940)-positive T-cell proliferation, survival, interleukin-2 (IL2; MIM 147680) production, and T-helper type-2 (Th2) development.[supplied by OMIM
Other Designations
CD28 antigen|CD28 antigen (Tp44)|T-cell-specific surface glycoprotein
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Interactome
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Pathway
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Disease
- Acute Disease
- Arthritis
- Asthma
- Behcet Syndrome
- Bronchiolitis
- Carcinoma
- Celiac Disease
- Cholangitis
- Colon cancer
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