EGF (Human) Recombinant Protein
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Specification
Product Description
Human EGF recombinant protein with His tag expressed in Escherichia coli.
Sequence
MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRLE
Host
Escherichia coli
Theoretical MW (kDa)
6
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
Measured by the induction of 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity is approximately > 1.4 x 106 IU/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 8.0.
Storage Instruction
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. Avoid repeated freezing and thawing.
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Applications
Functional Study
The ED50 for this effect is 0.05-0.12 ng/mL, measured by the induction of 3T3 cells proliferation.SDS-PAGE
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Gene Info — EGF
Entrez GeneID
1950Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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