EGF (Human) Recombinant Protein
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More Files
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Surface Plasmon Resonance
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.16 nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human EGF (P01133-1, Asn971-Arg1023) partial recombinant protein with hFc tag at N-terminus expressed in HEK293 cells.
Sequence
Asn971-Arg1023
Host
Human
Theoretical MW (kDa)
32.52
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.16 nM as determined in SPR assay (Biacore T200). -
Gene Info — EGF
Entrez GeneID
1950Protein Accession#
P01133-1Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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Pathway
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Disease
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