Products

Product Description

Human CD276 (Q5ZPR3-2, Leu 29 - Pro 245 ; Pro 99 - Lys 330) partial recombinant protein with Fc, His tag at C-terminal expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Host

Human

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purification

Ni-sepharose purification

Purity

> 95% by SDS-PAGE

Activity

ELISA plate pre-coated by 2 ug/mL (100 uL/well) CD276 (Human) Recombinant Protein (Cat # P6786), mFc-His tagged protein can bind Anti-B7-H3 Neutralizing antibody in a linear range of 0.24-31.25 ng/mL.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

CD276 (Human) Recombinant Protein (Cat # P6786) was determined by SDS-PAGE with Coomassie Blue, showing a band at 70-75 kDa.

Recommend Usage

SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from PBS, pH 7.4 (5% - 19% Trehalose)

Storage Instruction

Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitute with deionized water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.

Note

Result of activity analysis