PD-1 (Human) Recombinant Protein
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Specification
Product Description
Human PD-1 (NP_005009.2, 25 a.a. - 168 a.a.) partial recombinant protein with His tag at C_terminus expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
30-40
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
Measured by the binding ability in a functional ELISA. Immobilized Recombinant Human PD-1 at 5 ug/mL (100 uL/well) can bind Recombinant Human PD-L1 with a linear range of 0.5-2 ug/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
PD-1 (Human) Recombinant Protein (Cat #P6774) was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — PDCD1
Entrez GeneID
5133Protein Accession#
Q15116Gene Name
PDCD1
Gene Alias
CD279, PD1, SLEB2, hPD-1, hPD-l
Gene Description
programmed cell death 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
Other Designations
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