PDCD1 (Human) Recombinant Protein
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Specification
Product Description
Human PDCD1 (Q15116, 25 a.a. - 167 a.a.) partial recombinant protein with His tag at C-terminus expressed in Expi293 cells.
Sequence
LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ
Host
Human
Theoretical MW (kDa)
17
Form
Liquid
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 12.7 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of FITC-Labeled Human PD-1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human FcRH5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS pH 7.4
Storage Instruction
Store at -80°C for 12 Month.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human PD-1, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-PD1 Antibody, hFc Tag with the EC50 of 12.7 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — PDCD1
Entrez GeneID
5133Protein Accession#
Q15116Gene Name
PDCD1
Gene Alias
CD279, PD1, SLEB2, hPD-1, hPD-l
Gene Description
programmed cell death 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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