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Last updated: 2022/5/22

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IL3RA (Human) Recombinant Protein BioActive

  • Catalog # : P6767
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  • Specification
  • Product Description:
  • Human IL3RA (NP_002174.1, 20 a.a. - 305 a.a.) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 145-150
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purification:
  • Ni-sepharose purification
  • Purity:
  • > 90% by SDS-PAGE
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IL3 at 4 ug/mL (100 uL/well) can bind recombinant Human IL3RA, the EC50 of Human IL3RA is 5.3 ug/mL.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6767
    IL3RA (Human) Recombinant Protein (Cat #P6767) was determined by SDS-PAGE with Coomassie Blue, showing a band at 145-150kDa.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4.
  • Storage Instruction:
  • Store the lyophilized protein at -20°C to -80°C for long term.
    After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 3563
  • Gene Name:
  • IL3RA
  • Gene Alias:
  • CD123,IL3R,IL3RAY,IL3RX,IL3RY,MGC34174,hIL-3Ra
  • Gene Description:
  • interleukin 3 receptor, alpha (low affinity)
  • Gene Summary:
  • The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq
  • Other Designations:
  • CD123 antigen,Interleukin-3 receptor,OTTHUMP00000022828,interleukin 3 receptor, alpha
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