Human MET (NP_000236.2, 956 a.a. - 1390 a.a.) M1250T mutant partial recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host:
Viruses
Form:
Liquid
Preparation Method:
Baculovirus expression system.
Purification:
Glutathione sepharose chromatography.
Purity:
75%
Activity:
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: Srctide, ATP: 100 uM.
Quality Control Testing:
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer:
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction:
Stored at -80°C. Aliquot to avoid repeated freezing and thawing.
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq