MET (Human) Recombinant Protein
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Specification
Product Description
Human MET (P08581, 25 a.a. - 932 a.a.) partial recombinant protein with His-Avi tag at C-terminus expressed in Expi293 cells.
Sequence
ECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGILSKYFDLIYVHNPVFKPFEKPVMISMGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHLHSEAVLCTVPNDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFT
Host
Mammals
Theoretical MW (kDa)
80 - 85
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293) expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
Immobilized Human c-MET, His & Avi Tag at 2.0 ug/mL (100 uL/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16 ug/mL determined by ELISA.
Quality Control Testing
SDS-PAGE under reducing condition
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/mL
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MET
Entrez GeneID
4233Protein Accession#
P08581Gene Name
MET
Gene Alias
AUTS9, HGFR, RCCP2, c-Met
Gene Description
met proto-oncogene (hepatocyte growth factor receptor)
Gene Ontology
HyperlinkGene Summary
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HGF receptor|OTTHUMP00000069168|SF receptor|met proto-oncogene|met proto-oncogene tyrosine kinase|oncogene MET|scatter factor receptor
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Interactome
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Pathway
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Disease
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