Human CSF3 (190 a.a.) full-lenth recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host:
Plants
Theoretical MW (kDa):
10.8
Reactivity:
Human
Form:
Lyophilized
Preparation Method:
Hordeum vulgare (barley) expression system
Purification:
Chromatography
Purity:
> 98% by SDS-PAGE
Endotoxin Level:
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kineticassay.
Activity:
Activity has been measured by cell-based proliferation assay using mouse NFS-60 cells. The ED50 is typically < 0.1 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Storage Buffer:
Lyophilized from PBS, pH 7.2
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq