CSF3 (Human) Recombinant Protein
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Specification
Product Description
Human CSF3 (Q8N4W3, 27 a.a. - 200 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MHSALWTVQEATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP
Host
Escherichia coli
Theoretical MW (kDa)
18.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/μg of protein by gel clotting method
Activity
ED50 < 0.1 ng/mL, measured by a cell proliferation assay of M-NFS-60 cells, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
PAGE -
Applications
Functional Study
SDS-PAGE
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Gene Info — CSF3
Entrez GeneID
1440Protein Accession#
Q8N4W3Gene Name
CSF3
Gene Alias
G-CSF, GCSF, MGC45931
Gene Description
colony stimulating factor 3 (granulocyte)
Omim ID
138970Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164327|colony stimulating factor 3|filgrastim|granulocyte colony stimulating factor|lenograstim|pluripoietin
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