STK10 (Human) Recombinant Protein
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Specification
Product Description
Human STK10 (BAA35073.1, 1 a.a. - 968 a.a.) full-length recombinant protein expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
113
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
90 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Moesin-derived peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — STK10
Entrez GeneID
6793Protein Accession#
BAA35073.1Gene Name
STK10
Gene Alias
LOK, PRO2729
Gene Description
serine/threonine kinase 10
Omim ID
603919Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq
Other Designations
lymphocyte-oriented kinase
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Interactome
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Disease
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